Needletail

Needletail is a MIT-licensed, minimal-copying FASTA/FASTQ parser and k-mer processing library for Rust.

The goal is to write a fast and well-tested set of functions that more specialized bioinformatics programs can use. Needletail's goal is to be as fast as the readfq C library at parsing FASTX files and much (i.e. 25 times) faster than equivalent Python implementations at k-mer counting.

Example

extern crate needletail;
use needletail::{parse_fastx_file, Sequence, FastxReader};

fn main() {
    let filename = "tests/data/28S.fasta";

    let mut n_bases = 0;
    let mut n_valid_kmers = 0;
    let mut reader = parse_fastx_file(&filename).expect("valid path/file");
    while let Some(record) = reader.next() {
        let seqrec = record.expect("invalid record");
        // keep track of the total number of bases
        n_bases += seqrec.num_bases();
        // normalize to make sure all the bases are consistently capitalized and
        // that we remove the newlines since this is FASTA
        let norm_seq = seqrec.normalize(false);
        // we make a reverse complemented copy of the sequence first for
        // `canonical_kmers` to draw the complemented sequences from.
        let rc = norm_seq.reverse_complement();
        // now we keep track of the number of AAAAs (or TTTTs via
        // canonicalization) in the file; note we also get the position (i.0;
        // in the event there were `N`-containing kmers that were skipped)
        // and whether the sequence was complemented (i.2) in addition to
        // the canonical kmer (i.1)
        for (_, kmer, _) in norm_seq.canonical_kmers(4, &rc) {
            if kmer == b"AAAA" {
                n_valid_kmers += 1;
            }
        }
    }
    println!("There are {} bases in your file.", n_bases);
    println!("There are {} AAAAs in your file.", n_valid_kmers);
}

Installation

Needletail requires rust and cargo to be installed. Please use either your local package manager (homebrew, apt-get, pacman, etc) or install these via rustup.

Once you have Rust set up, you can include needletail in your Cargo.toml file like:

[dependencies]
needletail = "0.6.0"

To install needletail itself for development:

git clone https://github.com/onecodex/needletail
cargo test  # to run tests

Python

Documentation

For a real example, you can refer to test_python.py.

The python library only raise one type of exception: NeedletailError.

There are 2 ways to parse a FASTA/FASTQ: one if you have a string (parse_fastx_string(content: str)) or a path to a file (parse_fastx_file(path: str)). Those functions will raise if the file is not found or if the content is invalid and will return an iterator.

from needletail import parse_fastx_file, NeedletailError, reverse_complement, normalize_seq

try:
    for record in parse_fastx_file("myfile.fastq"):
        print(record.id)
        print(record.seq)
        print(record.qual)
except NeedletailError:
    print("Invalid Fastq file")

A record has the following shape:

class Record:
    id: str
    seq: str
    qual: Optional[str]

    def is_fasta(self) -> bool
    def is_fastq(self) -> bool
    def normalize(self, iupac: bool)

Note that normalize (see https://docs.rs/needletail/0.4.1/needletail/sequence/fn.normalize.html for what it does) will mutate self.seq. It is also available as the normalize_seq(seq: str, iupac: bool) function which will return the normalized sequence in this case.

Lastly, there is also a reverse_complement(seq: str) that will do exactly what it says. This will not raise an error if you pass some invalid characters.

Building

To work on the Python library on a Mac OS X/Unix system (requires Python 3):

pip install maturin

# finally, install the library in the local virtualenv
maturin develop --cargo-extra-args="--features=python"

To build the binary wheels and push to PyPI

# The Mac build requires switching through a few different python versions
maturin build --features python --release --strip

# The linux build is automated through cross-compiling in a docker image
docker run --rm -v $(pwd):/io ghcr.io/pyo3/maturin:main build --features=python --release --strip -f
twine upload target/wheels/*

Releasing A New Version

There is a Github Workflow that will build Python wheels for macOS (x86 and ARM) and Ubuntu (x86). To run, create a new release.

Getting Help

Questions are best directed as GitHub issues. We plan to add more documentation soon, but in the meantime "doc" comments are included in the source.

Contributing

Please do! We're happy to discuss possible additions and/or accept pull requests.

Acknowledgements

Starting from 0.4, the parsers algorithms is taken from seq_io. While it has been slightly modified, it is mainly coming from that library. Links to the original files are available in src/parser/fast{a,q}.rs.