#bioinformatics #graph

bin+lib gfautil

Command line tools for working with GFA files and related formats

5 unstable releases

new 0.3.2 Nov 19, 2020
0.3.1 Nov 12, 2020
0.3.0 Oct 17, 2020
0.2.0 Oct 8, 2020
0.1.0 Aug 18, 2020

#59 in Science

29 downloads per month

MIT license


Rust gfautil

Command line tool for various operations on GFA and related files.


Install with cargo:

cargo install gfautil

Or clone and build it:

git clone https://github.com/chfi/rs-gfa-utils.git
cd rs-gfa-utils
cargo build --release

The compiled binary will be located at target/release/gfautil.

$ gfautil
gfautil 0.3.2

    gfautil [FLAGS] [OPTIONS] -i <input GFA file> <SUBCOMMAND>

        --debug      Show debug messages
    -h, --help       Prints help information
        --info       Show info messages
        --quiet      Show no messages
    -V, --version    Prints version information

    -i <input GFA file>
    -t, --threads <threads>    The number of threads to use when applicable. If omitted, Rayon's default will be used,
                               based on the RAYON_NUM_THREADS environment variable, or the number of logical CPUs

    gaf2paf         Convert a file of GAF records into PAF records
    gfa2vcf         Output a VCF for the given GFA, using the graph's ultrabubbles to identify areas of variation
    help            Prints this message or the help of the given subcommand(s)
    id-convert      Convert a GFA with string names to one with integer names, and back
    snps            Given a reference path from the GFA, by name, find and report the SNPs for all other paths
                    compared to the reference.
    subgraph        Generate a subgraph of the input GFA


Given a GAF file, and the GFA used to create it, output a PAF file derived from the GAF records. For every path segment in each GAF record, a corresponding PAF record is produced.

Convert example.gaf, via example.gfa, with output on stdout:

gfautil -i ./example.gfa gaf2paf --gaf ./example.gaf

Save output to out.paf:

gfautil -i ./example.gfa gaf2paf --gaf ./example.gaf -o out.paf


Find the ultrabubbles in the input GFA, then use those to identify variants. For each ultrabubble, the section covered by the bubble is extracted from each embedded path. Those sub-paths are then compared pairwise.

The -u option can be used to load the ultrabubbles from a file (output by the ultrabubbles command) instead of computing them.

Currently the variant identification is mostly based on the nodes that make up each path, and only barely takes the sequences into account.

Outputs is in the VCF format, on stdout.

gfautil -i ./example.gfa gfa2vcf

There's a setting to skip comparing a pair of paths if their orientations at the start and end of the bubble don't match:

gfautil -i ./example.gfa gfa2vcf --no-inv

Loading the list of ultrabubbles from a file:

gfautil -i ./example.gfa gfa2vcf -u example.ultrabubbles

Identify SNPs in GFA against reference path

Given the name of a path in the input GFA to use as reference, identify SNPs among all other paths, using either a list of ultrabubbles constructed using the gfautil ultrabubbles command, or a list of SNP positions.

Outputs a tab-delimited list in the format:

<query-path-name>\t<reference base>\t<reference pos>\t<query base>\t<query pos>

SNP positions can be provided as a list in the arguments to gfautil:

gfautil --debug -t 8 -i ./example.gfa snps --ref "reference path name" --snps 1234 5677 1> example.gfa.snps

SNP positions can also be provided as a file, with one position per line:

gfautil --debug -t 8 -i ./example.gfa snps --ref "reference path name" --snps-file ./positions.txt 1> example.gfa.snps

Using ultrabubbles from a file:

gfautil -i ./example.gfa snps --ref the_path -u example.bubbles


Return a subgraph of the given GFA. Provide either a list of segment names, or a list of path names. If segment names are provided, the resulting subgraph will include the lines that contain at least one of those segments. If path names are provided, the segments in the given paths are used instead.

gfautil -i example.gfa subgraph segments --names s1 s2 s3
cat names.txt
gfautil -i example.gfa subgraph segments --file names.txt
gfautil -i example.gfa subgraph paths --names p1 p2


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